Aperio imagescope

Leica Biosystems Imaging, Inc. 2020... Page 8: Introduction Aperio ImageScope DX viewer For a complete description of this system and the workflow for using it, refer to the Aperio AT2 DX System Overview. The monitors provided with the Aperio AT2 DX System have been calibrated at the factory and may require periodic re-calibration in the field by authorized Leica Biosystems technicians. Page 9: Intended Use The Aperio AT2 DX System is composed of the Aperio AT2 DX scanner, the Aperio ImageScope DX review application and display. The Aperio AT2 DX System is for creation and viewing of digital images of scanned glass slides that would otherwise be appropriate for manual visualization by conventional light microscopy. Page 10: Symbols And Warnings Class A computing device (pursuant to Subpart B of Part 15 FCC Rules), which are designed to provide reasonable protection against such interference when operated in a commercial environment. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 11: Instrument Warnings Installation CAUTION: Two people are required for lifting the scanner. Installation and setup of the scanner should only be performed by a trained Leica Biosystems Technical Services representative. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 12: Startup And Shutdown System Startup To start the Aperio AT2 DX scanner: Press the power button on the front panel of the Aperio AT2 DX Control Station. When the Windows login screen appears, log in with the Windows user name and password. The default user name and the password were provided to your administrator on installation. We recommend that the administrator change the user name and password after installation. Page 13: Calibration And Verification Chapter 2: Startup and Shutdown Calibration and Verification The Aperio AT2 DX scanner is calibrated and verified at the factory before shipment. During installation, a Leica Biosystems technician also performs calibration and operational verification. We recommend annual preventive maintenance, performed by Leica Biosystems Technical Services, to make sure the scanner is operating correctly. Page 14: Preparing For Scanning Preparing for Scanning This chapter contains information on preparing

Sections of this chapter. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 28: Using Snapshots If the image has already been scanned, a small magnifying glass appears at the top left of the image image. Click it to open the scanned image in Aperio ImageScope DX. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 29: Working With Focus Points You can automatically place focus points in two ways on the slide view window. Use the Number of Focus Points slider to place a small number or large number of focus points. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 30: Calibration The scanner is calibrated and verified at the factory before shipment. No further manual calibration or verification is required by the customer. However, we recommend annual preventive maintenance, performed by Leica Biosystems Technical Services, to make sure the scanner is operating correctly. Page 31: Z-Stack Scanning Z-Stack Scanning The Aperio AT2 DX can create multiple digital images of slide tissue scanned at different focal depths, creating a 3D image that you can visually navigate through much as a microscope user can navigate through different tissue focal depths by using the microscope objective fine and coarse adjustments. Page 32: About The Z-Stack Scanning Window When satisfied with the settings, click Apply to apply the settings to the current slide and click the red X at the top of the window to close it. Click Scan on the slide view window to scan the slide. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 33: Advanced Z-Stack Settings Type a name for the slide setting. Click Save. Viewing Z-Stacks For information on viewing the 3-dimensional z-stack image, refer to the Aperio ImageScope DX User’s Guide. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 34: Image Quality Review Data Entry Data entry is automated using barcodes. When a glass microscope slide is scanned by the Aperio AT2 DX scanner, a barcode on the

On the Magnification icon.) Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 21: Batch View To select all slides in the batch view, click at the top of the window. To unselect all slides in the batch view, click at the top of the window. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 22: Quick Reference Selects all slides that Marks all selected Automatically sets indicator slides from scan failed to scan slides for rescanning focus points batch Selects z-stack scanning. Selects magnification of scan Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 23: Viewing Scan Status Eject each rack on the Console window as discussed in “Viewing Scan Status” on page 23 and replace the racks with loaded racks. Click the Scan button: Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 24 To see the slide in even more detail, click the magnifying glass at the top corner of the green box (which indicates the scanned area) to open the image in Aperio ImageScope DX. To return to the previous view, click the back arrow at the top of the page. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 25: Fine-Tuning Batch Scanning To place the rack in loading position, move the cursor to the bottom of the rack in rack view and click the Eject button: Then remove the rack, re-load it or replace it with another loaded slide rack, and place it back in the scanner. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 26 Magnification icon. Add name or Optionally, you can name one or more selected slides and give them a description by using the description Name and Description fields. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 27: Manually Scanning A Slide For details on the icons in the Manual Setup section shown on the slide view window above, see the following

Manuals Brands Leica Manuals Laboratory Equipment Aperio AT2 DX User manual Contents Table of Contents Troubleshooting Bookmarks Quick Links Aperio AT2 DXUser's GuideMAN-0351, Revision B | 13 April 2020 Need help? Do you have a question about the Aperio AT2 DX and is the answer not in the manual? Questions and answers Related Manuals for Leica Aperio AT2 DX Summary of Contents for Leica Aperio AT2 DX Page 1 Aperio AT2 DX User’s Guide MAN-0351, Revision B | 13 April 2020... Page 2 Copyright © 2019-2020 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH. Aperio is a trademark of the Leica Biosystems group of companies in the USA and optionally in other countries. Other logos, product and/or company names might be trademarks of their respective owners. Page 3 Tel: 0080052700527 (toll free) In-country Tel: +31 70 413 21 00 Danmark: Email: [email protected] Tel: 0080052700527 (toll free) In-country Tel: +45 44 54 01 01 Email: [email protected] Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 4 Tel: 0080052700527 (toll free) In-country Tel: +44 1908 577 650 Email: [email protected] USA: Tel: +1 (toll free) (844) 534-2262 Direct International Tel: +1 760 539 1150 Email: [email protected] Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 5: Table Of Contents Scanner Controls ..........................17 Scanning Parameters ........................18 Starting the Console ........................18 Barcode Requirement ........................18 Manually Entering a Barcode ......................19 Multiple Slide Scanning Using the AutoLoader ..................19 Replacing a Rack ........................19 Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 6 Perform a Presnap ..........................40 Set Line Camera Gains ........................40 Clean the Objective .........................41 Removing the Objective ........................41 Installing the Objective ........................41 Clean the Scanner ..........................42 Cleaning the Scanner Casing ......................42 Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 7 AutoLoader Specifications ........................56 Scanner Control Workstation Software Specifications ................57 System Requirements ........................57 Environment Specifications .......................57 Aperio AT2 DX Safety Instructions ................58 Index ............................. 60 Symbols..........................63 Aperio AT2 DX User’s Guide, Revision B ©

Handling it. If you are removing the objective to clean it, store the objective in the objective case supplied by Leica Biosystems when it is outside of the scanner. Page 42: Clean The Scanner Turn off power to the scanner and unplug the power cord before placing your hands inside the scanner. Using proper safety protection and procedures, pick up and wipe up the visible pieces of any broken slides. Please contact Leica Biosystems Technical Services for advice before operating the scanner. Page 43: Cleaning The Autoloader Racks Open the AT2 DX covers to gain access to the inside of the scanner. Locate the slide-in-rack sensor amplifier which is to the right of the AutoLoader cover. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 44 OTHER BUTTONS ON THE AMPLIFIER COULD PUT THE SCANNER INTO A STATE THAT CAN ONLY BE REMEDIED BY A SERVICE TECHNICIAN VISIT. Locate the Set button on the amplifier (marked in the illustration). The button is labeled “Set.” Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 45: Change The Aperio Service Account Password And Update Apservice Enter the new password and repeat it in the next text box. Click the arrow to proceed. Now you must go to the next section to update the service credentials. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 46: Updating The Aperio Service Credentials In the search box at the bottom of the pane, type services.msc. On the Services window, locate ApService: Right click on it and select Properties from the context menu. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 47 On the Log On tab, enter and confirm the password you used for the Aperio service account in “Changing the Aperio Service Account Password” on page 45. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 48 Click Apply. You see a warning that the changes won’t take effect until you stop and restart the service. Click OK

For scanning slides with the Aperio AT2 DX scanner. Training Leica Biosystems Imaging offers on-site training courses. Contact Leica Biosystems Imaging Customer Support for information about training options. Slide Preparation Tips When preparing slides for scanning, you must use the procedures required by the vendors of the stains and reagents you are using. Page 15: Coverslips Below are examples of a slide on which multiple labels cause the slide to exceed the allowable maximum thickness and a slide in which the label is peeling away from the slide: Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 16: Glass Slide Quality Control Glass Slide Quality Control It is the responsibility of the lab to verify the quality of the tissue preparation, the mechanics of a slide and the staining. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 17: Scanning Slides Scanning Slides This chapter contains information on scanning slides with the Aperio AT2 DX scanner. CAUTION: Keep hands clear of the mechanical stage assemblies when the scanner is operating. Scanner Controls Several of the controls on this scanner not labeled below are used in other configurations of this scanner, but are not used as part of an Aperio AT2 DX System. Page 18: Scanning Parameters QR Code Code 128 If the Aperio AT2 DX scanner is unable to interpret the barcode on the label of your slide, the slide will appear on the Console window in red to indicate the scan failed. In this case, inspect the slide to see if that area of the slide needs to be cleaned. Page 19: Manually Entering A Barcode Place a slide into the slide tray with coverslip up and label oriented to the front. For more information on scanning from the slide tray, see “Manually Scanning a Slide” on page 27. Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020... Page 20: Basic Concepts The dot to the left of each slide indicates the scan magnification selected for that slide. (The colors match the magnification levels shown